Ghrelin参与FSH对牛卵丘细胞体外增殖的调节

doi:10.11843/j.issn.0366-6964.2013.08.009

Abstract

Abstract:

To explore the possible effects of Ghrelin and certain relationship between Ghrelin and FSH (Follicle-stimulating hormone) in regulating the proliferation of cumulus cell freed from bovine cumulus-oocyte complex (COCs) in vitro maturation process, the Ghrelin mRNA levels within bovine cumulus cells varied depending on the oocyte maturation stage, the effect of FSH and FSHBI (FSH binding inhibitor) on Ghrelin mRNA expression and the effect of FSH and Ghrelin receptor antagonist (［D-Lys³］-GHRP-6) on regulating the expression of PCNA, BCL-2 and BAX mRNA at 8 h in vitro were detected by relative real-time RT-PCR experiments. And cumulus cell proliferative activity affected by FSH and ［D-Lys³］-GHRP-6 was determined by cell proliferation activity assay technology during bovine COCs maturation. The results showed that the expression of Ghrelin mRNA within cumulus cell was the highest at 8 h and the expressed difference among 8, 0 and 24 h was significant (P<0.05). The expression of Ghrelin mRNA in cumulus cell was prominently elevate when addition of 0.01 IU·mL^-1 FSH (P<0.05), while the Ghrelin mRNA levels was significantly decrease affected by 400 ng·mL^-1 FSHBI. The expression of PCNA and BCL-2 genes and the proliferative activity of cumulus cell were observably promoted by 0.01 IU·mL^-1 FSH (P<0.05), but were markedly reduced after adding ［D-Lys³］-GHRP-6 (P<0.05). The results suggested that Ghrelin involves in regulating cumulus cell proliferation triggered by FSH and that this research could help in understanding the mechanisms behind follicular development regulated by FSH.

CLC Number:

S826
S814.8

MI Yan, ZHANG Ling-li, LI Hai-jun, DU Chen-guang, CAO Gui-fang, WANG Xiu-mei. Ghrelin Involvements in FSH Induced Bovine Cumulus Cell Proliferation in vitro[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2013, 44(8): 1236-1243.

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Ghrelin Involvements in FSH Induced Bovine Cumulus Cell Proliferation in vitro

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